Project

Part:BBa_K3113209:Design

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-09-27)


pCAG_CD63_Ser161_6xHis_HiBit_P9SN


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimised to function in mammalian cells.


Source

CAG promoter, 6xHis: 6x repeated histidine sequence included in an extracellular loop of CD63, CD63: exosomal membrane protein, HiBit: small part of nanoluc luciferase, MCP: MS2 bacteriophage coat protein

References